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P3338

Sigma-Aldrich

Prostate Specific Antigen from human semen

buffered aqueous solution

Synonym(s):

Cancer-associated PSA, PSA

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32

Quality Level

UniProt accession no.

storage temp.

2-8°C

Gene Information

human ... KLK3(354)

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General description

Prostate specific antigen (PSA) is a 28.43 kDa single-chain glycoprotein containing 237 amino acids, encoded by the kallikrein gene mapped to human chromosome 19. It is specifically expressed by human prostate tissue and is one of the highly abundant proteolytic enzyme expressed in the seminal fluid.

Application

Prostate Specific Antigen from human semen has been used:
  • in microarray sandwich assay
  • in the preparation of sandwich immunosensor
  • as a target protein to test composite organic−inorganic nanoparticle (COIN) and Alexa conjugates in a plate-binding assay

Biochem/physiol Actions

Prostate Specific Antigen (PSA), a glycoprotein of the glandular kallikrein family, is a serine protease with chymotrypsin-like enzymatic activity. Circulating PSA exists in free and protease inhibitor-bound forms. Studying the ratios of free to inhibitor-bound forms may prove valuable in the diagnosis of prostate cancer.
Prostate-specific antigen (PSA) is an androgen-regulated serine protease specifically produced by prostatic tissue and is secreted into the lumen. PSA in semen cleaves semenogelins present in the seminal coagulum. Total PSA levels are increased in serum of prostate cancer (PCa) patients. PSA functions as a potential biomarker for PCa. Serum protease inhibitors such as α1-antichymotrypsin (α1-ACT), α2-macroglobulin (α2-M), and other acute-phase proteins form irreversible complexes with circulating PSA and hinder proteolytic activity of PSA.

Packaging

Package size based on protein content

Quality

Highly purified

Physical form

Solution in phosphate buffered saline, contains 0.05-0.1% sodium azide with 50% glycerol

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Chun-Jen Hsiao et al.
Disease markers, 2016, 8915809-8915809 (2016-04-12)
Glycans of prostate-specific antigen (PSA) in prostate cancer were found to be different from that in benign disease. It is difficult to analyze heterogeneous PSA glycoforms in each individual specimen because of low protein abundance and the limitation of detection
Pt nanoparticle label-mediated deposition of Pt catalyst for ultrasensitive electrochemical immunosensors
Zhang J, et al.
Biosensors And Bioelectronics, 26(2), 418-423 (2010)
Usefulness of total PSA value in prostate diseases diagnosis
Prcic A, et al.
Acta Informatica Medica, 24(3), 156-156 (2016)
Agnieszka Stolarczyk et al.
Sensors (Basel, Switzerland), 22(4) (2022-02-27)
The detection of chemicals is a fundamental issue of modern civilisation, however existing methods do not always achieve the desired sensitivity. Preconcentrators, which are devices that allow increasing the concentration of the intended analyte via e.g., adsorption/desorption, are one of
Barry Lutz et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 56(4), 371-379 (2007-12-12)
Surface-enhanced Raman scattering (SERS) nanoparticles are emerging as a new approach for optical detection of biomolecules. In a model assay in formalin-fixed paraffin-embedded (FFPE) prostate tissue sections, we detect prostate-specific antigen (PSA) using antibody (Ab) conjugated to composite organic-inorganic nanoparticles

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