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MSP09

Sigma-Aldrich

Membrane Scaffold Protein 1E3D1 D73C

recombinant, expressed in E. coli, Cysteine substituted at position 73

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.26

recombinant

expressed in E. coli

Assay

≥90% (SDS-GE)

form

buffered aqueous solution

shipped in

ambient

storage temp.

−20°C

General description

Nanodisc technology is an approach to render membrane proteins soluble in aqueous solutions in a native-like bilayer environment, where the membrane proteins remain stable and active. The Nanodisc concept is derived from high density lipoprotein (HDL) particles and their primary protein component, apolipoprotein. The Nanodisc is a non-covalent structure of phospholipid bilayer and membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1).

The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The MSP1E3D1 D73C variant of MSP1 differs from MSP1 in the following facets:
  • It deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence3 (which is known separately as MSP1D1).
  • It repeats the Helix 4 (H4), Helix 5 (H5) and Helix 6 (H6) sequences of the original MSP1 sequence between the parent Helix 6 (H6) and Helix 7 (H7) segments of MSP1D1.
  • It substitutes a cysteine (C) residue for an aspartic acid (D) residue in the Helix 2 (H2) portion of the protein, at position 73 of the original native A-1 sequence.
  • The initial histidine-tag is one amino acid shorter.

Application

For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.

Legal Information

Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.
  • 7,691,414 Membrane scaffold proteins
  • 7,662,410 Membrane scaffold proteins and embedded membrane proteins
  • 7,622,437 Tissue factor compositions and methods
  • 7,592,008 Membrane scaffold proteins
  • 7,575,763 Membrane scaffold proteins and tethered membrane proteins
  • 7,083,958 Membrane scaffold proteins
  • 7,048,949 Membrane scaffold proteins

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Membrane Scaffold Protein 1D1 recombinant, expressed in E. coli, buffered aqueous solution

Sigma-Aldrich

MSP01

Membrane Scaffold Protein 1D1

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The biotrophic pathogen Xanthomonas oryzae pv. oryzae (Xoo) produces a sulfated peptide named RaxX, which shares similarity to peptides in the PSY (plant peptide containing sulfated tyrosine) family. We hypothesize that RaxX mimics the growth-stimulating activity of PSY peptides. Root length
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Protocols

Membrane Scaffold Proteins: Nanodisc Formation

Nanodisc technology aids membrane protein solubilization, overcoming associated challenges in diverse protein classes.

Membrane Scaffold Proteins: Nanodisc Formation

Nanodisc technology aids membrane protein solubilization, overcoming associated challenges in diverse protein classes.

Membrane Scaffold Proteins: Nanodisc Formation

Nanodisc technology aids membrane protein solubilization, overcoming associated challenges in diverse protein classes.

Membrane Scaffold Proteins: Nanodisc Formation

Nanodisc technology aids membrane protein solubilization, overcoming associated challenges in diverse protein classes.

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