M5287
MES hydrate
≥99.5% (titration), pH 2.5-4.0 (0.5 M in H2O), BioXtra
Synonym(s):
2-Morpholineethanesulfonic acid hydrate, 2-(N-Morpholino)ethanesulfonic acid hydrate, 4-Morpholineethanesulfonic acid
About This Item
Recommended Products
product line
BioXtra
Assay
≥99.5% (titration)
form
crystalline powder
storage condition
dry at room temperature
impurities
Insoluble matter, passes filter test
ign. residue (900 °C)
≤0.05%
color
white
pH
2.5-4.0 (0.5 M in H2O)
useful pH range
5.5-6.7
pKa
6.1
solubility
H2O: 0.5 M, clear, colorless
water: 335.3 g/L at 20 °C
anion traces
chloride (Cl-): ≤0.005%
cation traces
Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.002%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.005%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%
absorption
≤0.020 at 280 in H2O at 0.5 M
≤0.025 at 260 in H2O at 0.5 M
application(s)
cell analysis
life science and biopharma
sample preparation
storage temp.
room temp
SMILES string
O.OS(=O)(=O)CCN1CCOCC1
InChI
1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
InChI key
MIIIXQJBDGSIKL-UHFFFAOYSA-N
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General description
MES hydrate buffer also exhibits high water solubility and minimal metal ion binding, making it an ideal choice for various applications:
- Cell Biology: MES hydrate buffer is often the preferred choice for buffering cell culture media due to its lower toxicity to cells compared to other buffers like Tris and phosphate.
- Protein Purification and Extraction: Its stability across a wide pH range and minimal metal ion binding make MES hydrate buffer a valuable asset in protein purification and extraction protocols.
- Gel Electrophoresis: MES hydrate buffer is also commonly used as a running buffer in denaturing gel electrophoresis, ensuring reliable results in protein separation and analysis.
Application
- As a component of extraction buffer in the extraction of the enzyme BACE1
- As a component of the reaction mixture for the measurement of 5,10-methenyltetrahydrofolate synthetase (MTHFS) enzyme activity in fibroblasts
- As a component of MES coupling buffer during the coupling of mAb or GST-fusion proteins to carboxylate-modified polystyrene latex (CML) beads
- to adjust the pH of the solution for the fluorescent labeling
- as a component of electrophoresis buffer
Features and Benefits
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Effective Buffering from pH 5.5-6.7 (25 °C) with a pKa of 6.1 (25 °C)
- Highly soluble in water
- Minimal metal ion binding
- Less toxic to cells than other buffers such as Tris and phosphate
- Stable in a wide pH range
- Low UV absorptivity
- Minimal reactivity
Preparation Note
Storage and Stability
Other Notes
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
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