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Sigma-Aldrich

Minimum Essential Medium Eagle, with Earl's salts

StableCell™, with Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Minimum Essential Medium Eagle, MEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

StableCell™ MEM, With Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
HEPES: no
Hanks’ salts (2% CO2): no
NaHCO3: yes
Earle’s salts (5% CO2): no
stable glutamine: yes
phenol red: yes
L-glutamine: no
glucose: yes

shipped in

ambient

storage temp.

2-8°C

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General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Application

Minimum Essential Medium Eagle has been used in murine fibroblast culture for in vitro cytocompatibility testing.

Other Notes

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln. It is supplemented with L-Ala-L-Gln dipeptide. This provides a more stable form of glutamine for cell culture. Free amino acid L-glutamine is known to be unstable in cell culture.

Legal Information

StableCell is a trademark of Sigma-Aldrich Co. LLC

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jaroslav Čapek et al.
Materials science & engineering. C, Materials for biological applications, 79, 550-562 (2017-06-21)
Recently, iron-based materials have been considered as candidates for the fabrication of biodegradable load-bearing implants. Alloying with palladium has been found to be a suitable approach to enhance the insufficient corrosion rate of iron-based alloys. In this work, we have
A novel high-strength and highly corrosive biodegradable Fe-Pd alloy: Structural, mechanical and in vitro corrosion and cytotoxicity study
Capek J, et al.
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Peter Minárik et al.
Materials science & engineering. C, Materials for biological applications, 73, 736-742 (2017-02-12)
Effect of processing by equal channel angular pressing (ECAP) on the degradation behaviour of extruded LAE442 magnesium alloy was investigated in a 0.1M NaCl solution, Kirkland's biocorrosion medium (KBM) and Minimum Essential Medium (MEM), both with and without 10% of
Eva Pruchova et al.
Bioelectrochemistry (Amsterdam, Netherlands), 127, 26-34 (2019-01-18)
Titanium biomaterials are widely used in the medical field due to their biocompatibility and excellent corrosion and mechanical resistance. However, these materials have no antibacterial properties. To obtain an antibacterial active surface, a nanostructure of Ti6Al4V alloy was created. This
Optimization of chemically defined cell culture media-replacing fetal bovine serum in mammalian in vitro methods
Van der VJ, et al.
Toxicology in vitro, 24(4), 1053-1063 (2010)
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