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G2N350

Sigma-Aldrich

GenElute™ Plant Genomic DNA Miniprep Kit

greener alternative

sufficient for 350 purifications

Synonym(s):

Plant Genomic DNA Miniprep, Gen Elute

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.55

usage

sufficient for 350 purifications

Quality Level

200

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA purification: suitable

greener alternative category

, Aligned

storage temp.

15-25°C

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General description

The GenElute Plant Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure DNA from a variety of plant species. The GenElute kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The purified genomic DNA is ready for immediate use in sensitive downstream applications such as:
  • PCR
  • restriction endonuclease digestion
  • cloning
  • Southern blots

Features and Benefits

  • Starting material: Up to 100 mg of plant tissue
  • Expected yield: Up to 20 μg
  • Elution volume: 100 - 200 μl
  • Time required: < 40 min
  • RNase treatment required: No

Principle

Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. Plant tissue is disrupted by grinding in liquid nitrogen and the DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. The purified DNA is greater than 20 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS
  • C2112Column Preparation SolutionSDS

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C

Supplementary Hazards

EUH032

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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V Roussel et al.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 111(1), 162-170 (2005-05-12)
A sample of 480 bread wheat varieties originating from 15 European geographical areas and released from 1840 to 2000 were analysed with a set of 39 microsatellite markers. The total number of alleles ranged from 4 to 40, with an
Daniel Lunn et al.
Plant physiology and biochemistry : PPB, 66, 91-97 (2013-03-19)
Fruit development entails a multitude of biochemical changes leading up to the mature green stage. During this period the cell wall will undergo complex compositional and structural changes. Inhibition of genes encoding elements of the machinery involved in trafficking to
Catherine Ravel et al.
Genome, 49(9), 1131-1139 (2006-11-18)
Information on single-nucleotide polymorphisms (SNPs) in hexaploid bread wheat is still scarce. The goal of this study was to detect SNPs in wheat and examine their frequency. Twenty-six bread wheat lines from different origins worldwide were used. Specific PCR-products were
Jochen C Reif et al.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 111(5), 838-845 (2005-07-22)
It has been claimed that the system that delivers the products of plant breeding reduces the diversity of cultivated varieties leading to an increased genetic vulnerability. The main goal of our study was to monitor the temporal trends in genetic
Catherine Ravel et al.
Functional & integrative genomics, 6(4), 310-321 (2006-03-29)
Wheat prolamin-box binding factor (WPBF) was shown to be an activator of Triticum aestivum L. storage protein genes. Three homoeologous genes encoding this transcription factor were isolated from a bacterial artificial chromosome genomic library and sequenced. The genes all have
View All Related Papers

Protocols

GenElute™ Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)

This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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