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F8649

Sigma-Aldrich

Formate Dehydrogenase from Candida boidinii

lyophilized powder, 5.0-15.0 units/mg protein

Synonym(s):

FDH, Formate:NAD+ oxidoreductase

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About This Item

CAS Number:
9028-85-7
Enzyme Commission number:
1.2.1.2 (BRENDA, IUBMB)
EC Number:
232-844-2
MDL number:
MFCD00081614
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

biological source

fungus (Candida boidinii)

Quality Level

200

form

lyophilized powder

specific activity

5.0-15.0 units/mg protein

composition

Protein, 5.0-20.0% biuret

storage temp.

2-8°C

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Application

Formate Dehydrogenase (FDH) is used for diagnostics in large scale industrial processes. Its used in the production of an unnatural amino acid, tert-L-leucine, a component of some HIV protease and matrix metalloprotease inhibitors.[1]

Biochem/physiol Actions

Formate dehydrogenase is an abundant enzyme from yeast Candida boidinii (CbFDH) that plays an important role in the energy supply of methylotrophic microorganisms and in the stress response of plants.[1]
Formate dehydrogenase is involved in the stress response of plants [2] and catalyzes the reduction of NAD+ to NADH.[3]

Unit Definition

One unit will oxidize 1.0 μmole of formate to CO2 per min in the presence of β-NAD at pH 7.6 at 37 °C.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCG4161
SLCF4323
SLCF0381
SLCD5061
SLCD2542

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Held in police custody.
A Skelt
Nursing times, 84(4), 50-52 (1988-01-02)
Do reactive oxygen species or does oxygen itself confer obligate anaerobiosis? The case of Bacteroides thetaiotaomicron.
Khademian, et al.
Molecular Microbiology, 114, 333-347 (2021)
Samrat Dutta et al.
The journal of physical chemistry. B, 116(1), 542-548 (2011-12-01)
Functionally relevant femtosecond to picosecond dynamics in enzyme active sites can be difficult to measure because of a lack of spectroscopic probes that can be located in the active site without altering the behavior of the enzyme. We have developed
Sabrina Witthoff et al.
Microbiology (Reading, England), 158(Pt 9), 2428-2439 (2012-07-07)
Here, we show that Corynebacterium glutamicum ATCC 13032 co-metabolizes formate when it is grown with glucose as the carbon and energy source. CO(2) measurements during bioreactor cultivation and use of (13)C-labelled formate demonstrated that formate is almost completely oxidized to
Sofia Marques da Silva et al.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 17(5), 831-838 (2012-04-25)
Desulfovibrio spp. are sulfate-reducing organisms characterized by having multiple periplasmic hydrogenases and formate dehydrogenases (FDHs). In contrast to enzymes in most bacteria, these enzymes do not reduce directly the quinone pool, but transfer electrons to soluble cytochromes c. Several studies
View All Related Papers

Questions

1–4 of 4 Questions  

  1. Could you please provide the uniprot accession code of the enzyme? Best regards

    1 answer

    1. The UniProt accession ID is O13437. Please access this link below:
      https://www.uniprot.org/uniprotkb/O13437/entry.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. What solvent can Product F8649, Formate dehydrogenase from Candida boidinii be  dissolved in?

    1 answer

    1. Product F8649, Formate dehydrogenase from Candida boidinii can be dissolved in 50 mM phosphate buffer, pH 7.5 to 8.0, or in double distilled water. Solubility is approximately 10 mg/ml.

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  4. How do you store Formate Dehydrogenase from Candida boidinii , product F8649, once it has been solublized?

    1 answer

    1. Aliquots of the enzyme Formate Dehydrogenase from Candida boidinii , product F8649, should be stable for at least 6 months when stored at -15 to -25°C, at +33°C for 1 day, and at +2 to +8°C for 7 days. Aliquots should be thawed only once.

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