A0295

Anti-Human IgA (α-chain specific)−Peroxidase antibody produced in goat

affinity isolated antibody

• Synonym(s): Goat Anti-Human IgA (α-chain specific)−HRP
• MDL number: MFCD00162417
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: goat
• Quality Level: 300
• conjugate: peroxidase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human
• concentration: 4-11 mg/mL
• technique(s): direct ELISA: 1:50,000; dot blot: 1:80,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100; western blot: suitable
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Anti-Human IgA (a-chain specific)-peroxidase antibody is isolated from goat anti-human IgA antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the alpha chain of human IgA.Goat antihuman IgA is conjugated to peroxidase and purified to remove unconjugated material. Specificity of the peroxidase conjugated anti-human IgA is determined by immunoelectrophoresis (IEP) and cross-reactivity of the conjugate is determined by enzyme linked immunoassays (ELISA).

Specificity

Anti-Human IgA (a-chain specific)-Peroxidase antibody produced in goat conjugate is specific for human IgA when tested against human IgA, IgG, IgM, Bence Jones Kappa and Lambda myeloma proteins. The antibody conjugate does not react with mouse or rat IgG.

Immunogen

Purified human IgA.

Application

Anti-Human IgA (α-chain specific) Peroxidase antibody produced in goat has been used in:

• Enzyme linked immunosorbent assays (ELISA) at dilution of 1:3,000 and 1: 1,600
• Tissue anti-transglutaminase antibody (tTG) assay at a dilution of 1:1,000
• Dot blot: a minimum working dilution of 1:80,000 was determined in a direct chemiluminescence assay using 10ng human IgA/dot. Luminol plus enhancer was used as substrate.
• Immunohistology using formalin-fixed, paraffin-embedded sections at a dilution of 1:100
• Transcytosis assay of dimeric IgA (D-IgA)
• Western Blotting

Biochem/physiol Actions

IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa and prevent the commensal microorganisms from invading the intestines.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT as preservative

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Storage and Stability

For continuous use, store at 2-8°C for a maximum of one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2