71386

Sodium chloride solution

BioUltra, for molecular biology, ~5 M in H₂O

• Synonym(s): Halite, NaCl, sodium chloride
• Linear Formula: NaCl
• CAS Number: 7647-14-5
• Molecular Weight: 58.44
• Beilstein: 3534976
• MDL number: MFCD00003477
• UNSPSC Code: 12352302
• PubChem Substance ID: 57652296
• NACRES: NA.25
• grade: for molecular biology
• form: liquid
• solubility: water: soluble

Properties

• grade: for molecular biology
• Quality Level: 100
• product line: BioUltra
• form: liquid
• concentration: ~5 M in H₂O
• technique(s): DNA extraction: suitable; LAMP: suitable
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• refractive index: n20/D 1.378
• pH: 5.0-7.0 (25 °C)
• solubility: water: soluble
• density: 1.186 g/mL (5 M in water)
• cation traces: Al: ≤1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: neat
• UV absorption: λ: 260 nm A_max: 0.02; λ: 280 nm A_max: 0.01
• suitability: suitable for molecular biology
• application(s): genomic analysis
• SMILES string: [Na+].[Cl-]
• InChI: 1S/ClH.Na/h1H;/q;+1/p-1
• InChI key: FAPWRFPIFSIZLT-UHFFFAOYSA-M

Description

General description

Sodium chloride is a vital nutrient that aids normal body functions in humans and animals. This chemical is commonly found in nature with a wide variety of biochemical applications, like intravenous fluids and density gradients. It is also used as a diluent to increase ionic strength in buffers or culture media. Sodium chloride is crucial for maintaining plasma osmolality and the extracellular fluid volume of the body. Excess NaCl leads to increased blood pressure, which has implications for cardiovascular and renal diseases. Sterile NaCl is a component of eye drop solutions. Traditionally, it has been used in high concentrations to preserve food since most bacteria cannot grow in high-salt conditions.

Application

Sodium chloride solution has been used as a component of:

• polyethylene glycol (PEG) buffer for the purification of double-tagged products
• NT2 buffer for the preparation of antibody-coated Protein A Sepharose (PAS) beads and immunoprecipitation of ribonucleoproteins (RNPs)
• chilled lysis buffer for the isolation of single nuclei from mice kidney

Features and Benefits

• Geologically stable
• Suitable for purification, precipitation, and crystallization

Other Notes

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Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves