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62288

Sigma-Aldrich

Lipase B Candida antarctica, recombinant from Aspergillus oryzae

powder, beige, ~9 U/mg

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About This Item

CAS Number:
9001-62-1
Enzyme Commission number:
3.1.1.3 (BRENDA, IUBMB)
EC Number:
232-619-9
MDL number:
MFCD00131509
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

recombinant

expressed in Aspergillus oryzae

Quality Level

100

form

powder

specific activity

~9 U/mg

mol wt

33 kDa

color

beige

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

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General description

Candida antarctica lipase B (CalB) is structurally similar to several other lipases and has a flexible lid.[1] It is made up of 317 amino acids and has a molecular weight of 33 kDa. Lipase B is a member of the alpha/beta hydrolase-fold family.[2]

Application

Lipase B Candida antarctica, recombinant from Aspergillus oryzae has been used:
  • as a standard to characterize the enzymatic properties of D5-CalB[3]
  • as an efficient biocatalyst to start the reaction to obtain (R)-ester via esterification of racemic secondary alcohol[4]
  • to investigate a “green” recycling route for polybutylene succinate (PBS) based on reactive extrusion[5]
  • to compare the esterification yield with adsorbed CaLB (aCaLB) and covalently immobilized CaLB (cCaLB)[6]

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Lipase B from Candida antarctica has been shown to be an effective catalyst for the synthesis of esters of ethyl D-glucopyranoside from fatty acids larger than octanoic acid.[7] It has also been found to catalyze a wide variety of organic reactions including many different regio- and enantio-selective syntheses.[8]
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Candida antarctica lipase B (CALB) possesses wide substrate specificity, high activity and high enantioselectivity, hence it is considered as a major enzyme in biotechnology. It also has the capability to perform in aqueous and non-aqueous reaction environments. CALB is used in transesterification, kinetic resolution and polymerization reactions.[2]

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 8.0 and 40°C (tributyrin, Cat. No. 91010, as substrate)

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL7188
BCCG5108
BCBP3380V
BCBW4983

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Sun-Ki Kim et al.
Biotechnology and bioengineering, 112(2), 346-355 (2014-09-04)
Escherichia coli is the best-established microbial host strain for production of proteins and chemicals, but has a weakness for not secreting high amounts of active heterologous proteins to the extracellular culture medium, of which origins belong to whether prokaryotes or
Structural behavior of Candida antarctica lipase B in water and supercritical carbon dioxide: A molecular dynamic simulation study
Housaindokht M R, et al.
Journal of Supercritical Fluids, 63, 180-186 (2012)
Simple amino acid tags improve both expression and secretion of Candida antarctica lipase B in recombinant Escherichia coli
Kim S K, et al.
Biotechnology and Bioengineering, 112(2), 346-355 (2015)
Preparation of passion fruit-typical 2-alkyl ester enantiomers via lipase-catalyzed kinetic resolution
Strohalm H, et al.
Journal of Agricultural and Food Chemistry, 58(10), 6328-6333 (2010)
A novel self-activation mechanism of Candida antarctica lipase B
Luan B and Zhou R
Physical Chemistry Chemical Physics, 19(24), 15709-15714 (2017)
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Questions

1–8 of 8 Questions  

  1. What is the assay procedure for this recombinant lipaseB from Aspergillus oryzae ? Could you please share the protocol ?

    1 answer

    1. This product has Quality Level of 100. Our Life Science product portfolio covers a large range of products for different applications. With regard to the designated application of the products, we have defined standard regulatory support levels for individual product groups. The support level reflects the application, the regulatory background and the related qualification status of the material. For your current inquiry, the requested method of analysis is not available, because it is not part of the defined standard support.

      For additional information regarding our M-Clarity™ Program, including a description of the different product classifications and typical product use for each MQ level, you may view the details by clicking on the following website link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/m-clarity-program

      Helpful?

  2. After dissolution of Lipase B in 0.05M potassium phosphate buffer (pH=7.5), at what temperature should it be stored?

    1 answer

    1. When reconstituted in 50 mM Tris-HCl, pH 8.0, the solution is stable for at least one month at 2–8 °C. Similar stability would be expected with other buffers at the appropriate pH.

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  3. Is Product 62288, Lipase B Candida antarctica, recombinant from Aspergillus oryzae, lyophilized?

    1 answer

    1. This lipase is a lyophilisate and may contain Gum arabic for adjustment of activity. The amount of Gum arabic in each vial is lot dependent.

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  4. What is Product 62288, Lipase B Candida antarctica, recombinant from Aspergillus oryzae, soluble in?

    1 answer

    1. Lipase B is expected to be soluble in water. We are able to dissolve the enzyme in water for the enzymatic assay. We also have tested the solubility of Lipase B in 0.05 M potassium phosphate buffer solution (pH=7.5) during quality control at a concentration of 10 mg/mL. According to the chemicals encyclopedia published by the Royal Society of Chemistry, almost all organic solvents decrease lipase activity, with the exception of petroleum ether.

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  5. What is the molecular weight of Lipase B Candida antarctica, recombinant from Aspergillus oryzae, Product 62288?

    1 answer

    1. Unfortunately, molecular weight information is unavailable at this time.

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  6. What solvent can Lipase B Candida antarctica, recombinant from Aspergillus oryzae, Product 62288, be dissolved in?

    1 answer

    1. Lipase B is expected to be soluble in water. We are able to dissolve the enzyme in water for the enzymatic assay. We also have tested the solubility of Lipase B in 0.05M potassium phosphate buffer solution (pH=7.5) during quality control at a concentration of 10mg/mL. According to the chemicals encyclopedia published by the Royal Society of Chemistry, almost all organic solvents decrease lipase activity, with the exception of petroleum ether.

      Helpful?

  7. How pure is Product 62288, Lipase B Candida antarctica, recombinant from Aspergillus oryzae?

    1 answer

    1. Purity is not determined for this product. As is the case with many of our enzymes, the product is assayed for enzymatic activity, not for purity.

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  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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