70186

EMB Agar

NutriSelect^® Plus, suitable for microbiology

• Synonym(s): Eosin Methylene Blue Agar
• UNSPSC Code: 41171606
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15.0 g/L ; dipotassium hydrogen phosphate, 2.0 g/L ; eosin Y, 0.4 g/L ; lactose, 10.0 g/L ; methylene blue, 0.065 g/L ; peptone, 10.0 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 7.1±0.2 (25 °C)
• application(s): agriculture; cleaning products; clinical testing; cosmetics; environmental; food and beverages; personal care; microbiology
• suitability: slightly selective and differential for Candida spp.; slightly selective and differential for Citrobacter spp.; slightly selective and differential for Enterobacter spp.; slightly selective and differential for Enterococcus spp.; slightly selective and differential for Escherichia coli; slightly selective and differential for Klebsiella spp.; slightly selective and differential for Proteus spp.; slightly selective and differential for Salmonella spp.; slightly selective and differential for Staphylococcus spp.; slightly selective and differential for coliforms; slightly selective and differential for enterobacteriaceae

Description

Application

A versatile solid medium for the differentiation of Eschericha coli and Aerobacter aerogenes, for identifcation of Candida albicans and for the identification of coagulase-positive Staphylococci.

Preparation Note

Suspend 37.5 g in 1 litre of distilled water. Bring to the boil to dissolve completely, sterilize by autoclaving at 121°C for 15 minutes. Cool to 60°C and shake the medium in order to oxidize the methylene blue and to suspend the precipitate.

Other Notes

Recommended by the American Public Health Association (APHA) and the United States Pharmacopeia (USP); Compendium of Methods for the Microbiological Examination of Foods; Enumeration of E. coli from lake water.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves