22092

Tryptic Soy Broth - Dehydrated Culture Media

NutriSelect^® Plus, powder, suitable for microbiology

• Synonym(s): CASO Broth, Soybean Casein digest Broth, TSB, Tryptone Soy Broth, CASO Broth, Casein Soya Broth, Soybean Casein digest Broth, TSB, Tryptone Soya Broth
• MDL number: MFCD00132536
• UNSPSC Code: 41171607
• NACRES: NA.74

Properties

• Agency: according to ISO 10273:2017; according to ISO 21871:2006
• Quality Level: 200
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: casein peptone (pancreatic), 17 g/L ; dipotassium hydrogen phosphate, 2.5 g/L ; glucose, 2.5 g/L ; sodium chloride, 5 g/L ; soya peptone (papain digest.), 3 g/L
• packaging: bottle of 500 g
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• color: light yellow
• final pH: 7.3±0.2 (25 °C)
• application(s): bioburden testing; cleaning products; clinical testing; cosmetics; environmental; food and beverages; pathogen testing; personal care; pharmaceutical; sample preparation; veterinary; water monitoring; microbiology
• suitability: nonselective for Bacillus spp.; nonselective for Bacteroides spp.; nonselective for Campylobacter spp.; nonselective for Candida spp.; nonselective for Escherichia coli; nonselective for Neisseria spp.; nonselective for Staphylococcus spp.; nonselective for Streptococcus spp.; nonselective for bacteria (General Media); nonselective for coliforms

Description

General description

Tryptic Soy Broth is a general-purpose culture medium for the isolation and cultivation of fastidious and non-fastidious microorganisms or the maintenance of stock culture. It is a complex medium suitable for the cultivation and isolation of a wide range of bacteria, yeasts, and molds. The formulation of the TSB medium contains a combination of casein and soy peptones supplying organic nitrogen, particularly amino acids, and long-chained peptides. Sodium chloride maintains the osmotic equilibrium, and the natural sugars from soy peptone are the energy sources.

Application

Tryptic Soy Broth is a medium that supports the luxuriant growth of many fastidious organisms without adding serum. It is used for confirmation of Campylobacter jejuni by motility test. It is suitable for the cultivation of both aerobic and anaerobic bacteria.

Features and Benefits

NutriSelect^® Plus are powdered culture media offering

• Quality control with growth promotion test according to industry standards
• Quality assurance acc. to ISO 9001
• Cost-efficient powdered media

Preparation Note

Suspend 30 g of dehydrated media in 1 litre of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.

Other Notes

Capability of selective media to detect heat-injured Shigella flexneri

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves