PGI-RO

Phosphoglucose Isomerase (PGI)

from yeast

• Synonym(s): glucose-6-phosphate isomerase
• Enzyme Commission number: 5.3.1.9 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: yeast
• Quality Level: 100
• form: suspension
• specific activity: ~350 units/mg protein (at 25 °C with fructose-6-P as the substrate.)
• packaging: pkg of 1 mL (10127396001 [2 mg]); pkg of 1 mL (10128139001 [10 mg])
• manufacturer/tradename: Roche
• optimum pH: 7.0-7.6
• storage temp.: 2-8°C

Description

General description

Phosphoglucose Isomerase is an enzyme which possesses diagnostic potential when fractionated by cellulose acetate electrophoresis. This cytosolic enzyme plays an important role during glycolysis and glucogenesis. It acts as an tumor-secreted cytokine and autocrine motility factor. It is used as neurotrophic factor, maturation factor and as serine proteinase inhibitor.[1]

Specificity

Phosphoglucose isomerase (PGI) is specific for G6P (K_m = 0.7 mM) and fructose-6-phosphate (F6P).

Application

Isomerization of ketoses to aldoses.

Quality

Contaminants: <0.01% F6P-K, GR, 6-PGDH and PGluM, each, <0.2% β-fructosidase

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Storage conditions (working solution): A solution of PGI in 20 mM Tris, pH 8.1, containing 0.2 mg BSA/ml, is stable approx. 1 week at 2 to 8 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): No data available
• Flash Point(C): No data available