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MABN328

Sigma-Aldrich

Anti-peptidyl-citrulline, clone F95 Antibody

clone F95, from mouse

Synonym(s):

Peptidyl-citrulline, Protein-bound citrulline

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

F95, monoclonal

species reactivity

mouse, rat, chicken, human

species reactivity (predicted by homology)

all (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgMκ

shipped in

wet ice

target post-translational modification

unmodified

General description

Deimination (citrullination) is a post translational modification in which the peptidyl-arginine deiminase family of enzymes convert the guanidino side chain of the amino acid arginine into a ureido group, yeilding peptidyl-citrulline. As a result, anti-peptidyl-citrulline antibodies should also cross react with peptidyl-homocitrulline, produced by the carbamylation of lysine, but not free citrulline, which is created in the urea cycle, or as a by-product of nitric oxide synthesis.

Immunogen

Citrullinated peptide consisting of 10 citrulline residues and a carboxyl Gly-Gly-Cys.

Application

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected peptidyl-citrulline in human cerebellum, and rat cerebellum tissue.
Western Blotting Analysis: A representative lot detected peptidyl-citrulline in CNS proteins from brains and spinal cord of EAE mice (Nicholas et al., 2005).
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
This Anti-peptidyl-citrulline, clone F95 Antibody is validated for use in Immunohistochemistry and Western Blotting and Immunocytochemistry for the detection of peptidyl-citrulline.

Quality

Evaluated by Immunohistochemistry in human cerebral cortex tissue.

Imunnohistochemistry Analysis: A 1:250 dilution of this antibody detected peptidyl-citrulline inhuman cerebral cortex tissue.

Target description

Various kDa calculated

Physical form

Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Michael F Criscitiello et al.
International journal of molecular sciences, 21(8) (2020-04-25)
The bovine immune system is known for its unusual traits relating to immunoglobulin and antiviral responses. Peptidylarginine deiminases (PADs) are phylogenetically conserved enzymes that cause post-translational deimination, contributing to protein moonlighting in health and disease. PADs also regulate extracellular vesicle
Caitlyn L Holmes et al.
PloS one, 14(5), e0217221-e0217221 (2019-05-29)
The antibody response to pertussis vaccination in rheumatoid arthritis is unknown, a concerning omission given the relatively low efficacy of the pertussis vaccine, a rise in pertussis infections, and a general increased susceptibility to infection in rheumatoid arthritis. Additionally, the
R Bright et al.
Journal of periodontal research, 53(1), 139-143 (2017-10-19)
Postranslational modification of proteins can lead to the production of autoantibodies and loss of immune tolerance. This process has been hypothesised to be a critical factor in the pathogenesis of rheumatoid arthritis. The objective of this study was to demonstrate
Sanna Turunen et al.
Arthritis research & therapy, 18(1), 239-239 (2016-10-22)
Seropositive rheumatoid arthritis (RA) is characterized by autoantibodies binding to citrullinated and homocitrullinated proteins. We wanted to study the expression patterns of these disease-associated protein forms and if the rheumatoid nodule and synovial tissue itself contain biologically active levels of
Marianne Engström et al.
Journal of translational medicine, 16(1), 214-214 (2018-08-02)
A relationship between rheumatoid arthritis (RA) and periodontitis has been suggested from findings that individuals with RA are prone to have advanced periodontitis and vice versa. In search of possible common pathogenetic features of these two diseases, we investigated the
View All Related Papers

Questions

  1. I recently purchased Anti-peptidyl-citrulline, clone F95 Antibody to check whether my protein (beta catenin) is citrullinated but have yet not managed to detect any band on western blot. How is Anti-peptidyl-citrulline, clone F95 Antibody used?

    1 answer

    1. Kindly review our Anti-Citrulline (Modified) Detection Kit product: 17-347B available at this link: https://www.emdmillipore.com/US/en/product/Anti-Citrulline-Modified-Detection-Kit,MM_NF-17-347B. Also kindly review the following citation that utilized the kit:

      Qu Y, Olsen JR, Yuan X, Cheng PF, Levesque MP, Brokstad KA, Hoffman PS, Oyan AM, Zhang W, Kalland KH, Ke X. Small molecule promotes β-catenin citrullination and inhibits Wnt signaling in cancer. Nat Chem Biol. 2018 Jan;14(1):94-101. doi: 10.1038/nchembio.2510. Epub 2017 Oct 30. PMID: 29083417.

      If additional assistance is required, please reach out to Technical Service so we may assist you directly.

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