The molar extinction coefficient of the product at 410 nm is estimated to be approximately 35,000 M-1 cm-1. This value is slightly lower than the 36,000 M-1 cm-1 (415 - 420 nm) reported in the publications below:
Appl. Environ. Microbiol. 2014, 80(24), 7484-7495
W.G. Biochem. J. 1975, 145, 93
ES004
ABTS Single Reagent, Blue green color,Horseradish Peroxidase Substrate (soluble)
500 ml ABTS Single Reagent, Blue green color, Horseradish Peroxidase Substrate (soluble) for ELISA.
Synonym(s):
Horseradish Peroxidase Substrate
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About This Item
UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NA.25
Pricing and availability is not currently available.
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form
liquid
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
λmax
410 nm
650 nm
General description
ABTS is considered a safe sensitive substrate for horseradish peroxidase based ELISA systems. The blue-green color produced by enzymatic activity can be quantitated at 405-410 nm. The reaction can be stopped by addition of acid and evaluated at unstopped wavelengths. The reagent system can be compared to TMB if the reaction is allowed to progress for 30 minutes. CHEMICON provides a room temperature stable single component ABTS reagent system for use in ELISA procedures. The proprietary non-organic formulation furnishes the researcher with a horseradish peroxidase detection system that can be employed as a kinetic or end point procedure evaluated at the same wavelength.
Application
500 ml ABTS Single Reagent, Blue green color, Horseradish Peroxidase Substrate (soluble) for ELISA.
Physical form
ABTS in a proprietary buffer with enhancer.
Color Code: Green
Storage and Stability
Maintain at room temperature (18-26ºC) for up to 6 months. For extended storage maintain at 2-8°C for up to 12 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Certificates of Analysis (COA)
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Mit welchem Extinktionskoeffizienten kann für dieses Produkt bei 410 nm gerechnet werden?
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