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Key Documents

ABC217

Sigma-Aldrich

Anti-GLI1 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Zinc finger protein GLI1, Glioma-associated oncogene, Oncogene GLI

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GLI1(2735)

General description

Zinc finger protein GLI1 is also called Glioma-associated oncogene and Oncogene GLI. GLI1 is a transcriptional activator that regulates specific genes during normal development. GLI1 also mediates SHH signaling (and therefore cell proliferation and differentiation) and possibly is involved in craniofacial, central nervous system, gastrointestinal tract, and digital development. GLI1 is expressed in the testis, myometrium, fallopian tube, and brain.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human GLI1.

Application

Anti-GLI1 Antibody is a highly specific rabbit polyclonal antibody, that targets Gli1 & has been tested in western blotting & IHC (Paraffin).
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blotting Analysis: A 1:500 dilution from a representative lot detected GL1 in A431, HEK293, HEPG2, and PC3 cells.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected GLI1 in human cerebellum and thalamus tissues.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected GLI1 in rat testis tissue.

Quality

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected GLI1 in 10 µg of A549 cell lysate.

Target description

~150 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Linkage

Replaces: MABC678

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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