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ABC10

Sigma-Aldrich

Anti-Megf10 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Multiple epidermal growth factor-like domains protein 10, Multiple EGF-like domains protein 10

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

technique(s)

immunofluorescence: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Megf10(70417)

General description

Multiple epidermal growth factor-like domains protein 10 (Megf10) is a type 1 transmembrane protein with multiple EGF-like extracellular domains. Megf10 is highly expressed in the brain, and plays an important role in phacocytosis of apoptotic cells. This role may be performed through the interaction of Megf10 with the ATP-binding cassette transporter (ABCA1) protein. Previous studies have suggested that Megf10 may be involved in the uptake of amyloid β peptides in the brain. Megf10 may therefore play a role Alzheimer’s disease.

Specificity

Other homologies: Rat (98% sequence homology) and Human (92% sequence homology).
This antibody recognizes Megf10 at the cytoplasmic domain.

Immunogen

Epitope: Cytoplasmic domain
Histidine-tagged recombinant protein corresponding to the cytoplasmic domain of mouse Megf10.

Application

Detect the Megf10 protein using this Anti-Megf10 validated for use in WB & IF.
Immunofluorescence Analysis: A representative lot was used by an independent laboratory in P7 caudal brain tissue and retina cells. Image courtesy from the laboratory of Dr. Joshua Sanes, Harvard University.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blot in mouse retina tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Megf10 on 10 µg of mouse retina tissue lysate.

Target description

~130 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse retina tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dai Shi et al.
International journal of molecular sciences, 24(2) (2023-01-22)
It has been hypothesised that inhalational anaesthetics such as isoflurane (Iso) may trigger the pathogenesis of Alzheimer's disease (AD), while the gaseous anaesthetic xenon (Xe) exhibits many features of a putative neuroprotective agent. Loss of synapses is regarded as one
Michael Surala et al.
EMBO reports, 25(5), 2348-2374 (2024-04-09)
Microglia sculpt developing neural circuits by eliminating excess synapses in a process called synaptic pruning, by removing apoptotic neurons, and by promoting neuronal survival. To elucidate the role of microglia during embryonic and postnatal brain development, we used a mouse
Hiroyuki Konishi et al.
The EMBO journal, 39(22), e104464-e104464 (2020-09-23)
Microglia are the principal phagocytes that clear cell debris in the central nervous system (CNS). This raises the question, which cells remove cell debris when microglial phagocytic activity is impaired. We addressed this question using Siglechdtr mice, which enable highly
Lingjie Li et al.
International journal of molecular sciences, 25(2) (2024-01-23)
Synapse loss is one of the most critical features in Alzheimer's disease (AD) and correlates with cognitive decline. Astrocytes mediate synapse elimination through multiple EGF-like domains 10 (MEGF10) pathways in the developing and adult brain to build the precise neural
Yin-Peng Chen et al.
Frontiers in molecular neuroscience, 9, 105-105 (2016-11-09)
Cytoplasmic polyadenylation element binding protein 3 (CPEB3) regulates target RNA translation in neurons. Here, we examined CPEB3 distribution and function in the mouse retina. CPEB3 is expressed in retinal neurons, including those located in the inner nuclear layer (INL) and

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