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Key Documents

AB1344

Sigma-Aldrich

Anti-Glucose Transporter GLUT-3 Antibody, CT

serum, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

Specificity

Recognizes Glut-3 in mouse and rat tissues. C terminal peptide used shows no homology to the human sequence.

Immunogen

Synthetic peptide corresponding to the C-terminus of mouse Glut-3 coupled to KLH. Product sold sold as AG617

Application

Anti-Glucose Transporter GLUT-3 Antibody, C-terminus is an antibody against Glucose Transporter GLUT-3 for use in ELISA & WB.
Western blotting: 1:1,000-1:5,000, using chemiluminescent detection. Detects a 45-48kDa band in membrane preps from rat brain. Slight differences in band sizes in different tissues; reported range is 45-55kDa.

Other detection methods may require a higher concentration of antibody.

ELISA: 1:10,000 - 1:50,000, using 50-100 ng of antigen/well.

Optimal working dilutions must be determined by end user.

Physical form

Liquid rabbit antiserum containing 0.05% sodium azide.

Storage and Stability

Store at -20°C for up to 12 months after date of receipt in undiluted aliquots. Avoid repeated freeze-thaw cycles.

Analysis Note

Control
The control peptide is available for absorbtion studies. Please see catalog number AG617.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Proceedings of the National Academy of Sciences of the United States of America, 97(18), 10236-10241 (2000-08-24)
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The Journal of biological chemistry, 278(8), 5828-5836 (2002-12-18)
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Hypoglycemia occurs frequently during intensive insulin therapy in patients with both type 1 and type 2 diabetes and remains the single most important obstacle in achieving tight glycemic control. Using a rodent model of hypoglycemia, we demonstrated that exposure to
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Testing null

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