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71340-M

Sigma-Aldrich

pCDFDuet-1 DNA - Novagen

Synonym(s):

Duet-1 DNA, Novagen DNA Vector, pCDFDuet-1 DNA

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About This Item

UNSPSC Code:
41106602
NACRES:
NA.51

form

liquid

Quality Level

manufacturer/tradename

Novagen®

storage condition

OK to freeze

Promoter

Promoter name: T7

selection

spectinomycin

storage temp.

−70°C

General description

pCDFDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet-1, pACYCDuet-1, and pRSFDuet-1 or pCOLADuet-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.

Application

pCDFDuet-1 DNA has been used as a vector for the expression of recombinant proteins in E-coli cells.

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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