This product is a 100ug/mL solution in 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl?, 1 µM ZnCl?, 0.05% BRIJ® 35 Detergent, 0.05% NaN?, pH 7.0. See the link below for a general protocol. The researcher would have to optimize for use specifically with collagen.
https://www.emdmillipore.com/US/en/product/MMP-8-Human-Neutrophil,EMD_BIO-444229#anchor_PDS
444229
MMP-8, Human Neutrophil
Synonym(s):
Matrix Metalloproteinase 8, Human Neutrophil Collagenase, Collagenase-2
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About This Item
Pricing and availability is not currently available.
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Assay
≥90% (SDS-PAGE)
Quality Level
form
liquid
specific activity
≥40 mU/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
foreign activity
other MMP, none detected
shipped in
wet ice
storage temp.
−70°C
General description
Native pro-MMP-8 from stimulated human neutrophils. MMP-8 is an N-linked, complex glycoprotein that preferentially hydrolyzes Type I collagen versus Types II and III. Activated MMP-8 is inhibited by TIMP-1. Requires activation just prior to use. MW 58800/44000.
Native pro-MMP-8 from stimulated human neutrophils. MMP-8 is an N-linked, complex glycoprotein that preferentially hydrolyzes Type I collagen versus Types II and III. Activated MMP-8 is inhibited by TIMP-1. Requires activation prior to use.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Other Notes
Deschamps, A. M., et al. 2005. Circulation111, 1166.
Mallya, S.K., et al. 1990. Biochemistry 29, 10628.
Marcy, A.I., et al. 1991. Biochemistry30, 6476.
Stricklin, et al. 1983. Biochemistry22, 61.
Mallya, S.K., et al. 1990. Biochemistry 29, 10628.
Marcy, A.I., et al. 1991. Biochemistry30, 6476.
Stricklin, et al. 1983. Biochemistry22, 61.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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MMP-8 Human Neutrophil in which buffer it can be dissolve. For checking activity of MMP with collagen.
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