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1.02439

Sigma-Aldrich

Eosin Y-solution 0.5% alcoholic

for microscopy

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About This Item

UNSPSC Code:
41116124
NACRES:
NA.41

Quality Level

form

liquid

IVD

for in vitro diagnostic use

transition temp

flash point 19.1 °C

density

0.8889 g/cm3 at 20 °C

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

General description

The Eosin Y-solution 0.5%, alcoholic - for microscopy, is a ready-to-use solution that can be used for routine staining in the hematoxylin-eosin staining process (H&E) of sample material of human origin. The H&E staining process is the most frequently used staining method for histological material. The staining mechanism behind the H&E staining is a physico-chemical process. In the first step, the positively charged nuclear dye (Mayer′s hematoxylin , Product number 1.09249, or Hematoxylin solution modified acc. to Gil III, Product number 1.05154) binds to the negatively charged phosphate groups of the nucleic acid of the cell nucleus. The nuclei will be dyed dark blue to dark violet. The second step is the counterstaining with negatively charged anionic Eosin Y, a xanthene dye. Eosin binds to the positively charged plasma proteins. Cytoplasma and intercellular substances are stained pink to red, while erythrocytes will appear with yellow to orange color.
The 500 ml bottle is sufficient for approx. 750 specimens. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

66.4 °F

Flash Point(C)

19.1 °C


Certificates of Analysis (COA)

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Taeyun Ku et al.
Nature methods, 17(6), 609-613 (2020-05-20)
We developed entangled link-augmented stretchable tissue-hydrogel (ELAST), a technology that transforms tissues into elastic hydrogels to enhance macromolecular accessibility and mechanical stability simultaneously. ELASTicized tissues are highly stretchable and compressible, which enables reversible shape transformation and faster delivery of probes
Matthias Schnetz et al.
Cancers, 12(3) (2020-02-29)
Accumulating evidence suggests that iron homeostasis is disturbed in tumors. We aimed at clarifying the distribution of iron in renal cell carcinoma (RCC). Considering the pivotal role of macrophages for iron homeostasis and their association with poor clinical outcome, we

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