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Key Documents

04-293

Sigma-Aldrich

Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8

clone 7F8, Upstate®, from mouse

Synonym(s):

NEU, NGL, HER2, TKR1, HER-2, c-erb B2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

7F8, monoclonal

species reactivity

mouse, human, canine

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_001005862.1
NM_004448.2

UniProt accession no.

P04626

shipped in

wet ice

target post-translational modification

phosphorylation (pThr686)

Gene Information

human ... ERBB2(2064)
mouse ... Erbb2(13866)

Specificity

Recognizes phosphorylated erbB2 on Threonine 686

Immunogen

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 686 in human erbB2

Application

Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8 is an antibody against phospho-erbB2 (Thr686) for use in ELISA & WB.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors

Kinases & Phosphatases

Quality

Routinely evaluated by immunoblot.

Target description

180 kDa

Physical form

Format: Purified
In 1 mL of PBS containing 0.09% sodium azide, PEG, sucrose and 50% glycerol.
Subsequent thiophilic adsorption and size exclusion chromatography

Storage and Stability

2 years at -20°C from date of shipment. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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X Ouyang et al.
Carcinogenesis, 19(11), 2013-2019 (1998-12-17)
Tumour cell growth may be accelerated by protein kinase C (PKC) agonists such as phorbol esters and receptor tyrosine kinases, but receptor tyrosine kinases are in turn desensitized to growth factors by PKC agonists. To clarify this apparent PKC bifunctionality
Human cancer cells exhibit protein kinase C-dependent c-erbB-2 transmodulation that correlates with phosphatase sensitivity and kinase activity
Ouyang, X, et al
The Journal of Biological Chemistry, 271, 21786-21792 (1996)
Natasha M Bourgeois et al.
Frontiers in cellular and infection microbiology, 14, 1264525-1264525 (2024-04-08)
Dengue is an arboviral disease causing severe illness in over 500,000 people each year. Currently, there is no way to constrain dengue in the clinic. Host kinase regulators of dengue virus (DENV) infection have the potential to be disrupted by

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