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I2002

Isocitric Dehydrogenase (NADP) from porcine heart

Name: Isocitric Dehydrogenase (NADP) from porcine heart
Brand: SIGMA

Type IV, buffered aqueous glycerol solution, 3-20 units/mg protein

Sinônimo(s):

threo-D_s-Isocitrate: NADP⁺ oxidoreductase, ICDH, IDH

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Sobre este item

Número CAS:

9028-48-2

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

200-289-5

MDL number:

MFCD00131420

Número CE:

1.1.1.42 (BRENDA, IUBMB)

Specific activity:

3-20 units/mg protein

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Propriedades

type

Type IV

Quality Segment

200

form

buffered aqueous glycerol solution

quality

≤ 0.2% of main activity is obtained with NAD as cofactor.

specific activity

3-20 units/mg protein

composition

Protein biuret

shipped in

wet ice

storage temp.

2-8°C

Descrição

Application

Isocitric dehydrogenase (IDH) has been used:

for in vitro activity assay to study the effects of thorium on citric acid cycle enzymes in the mitochondrial respiration pathway[1]
as a standard to measure the impact of temperature on nicotinamide diphosphate (NADP)-IDH activity
in isocitrate assay[2]

Biochem/physiol Actions

Isocitrate dehydrogenase (IDH) is a homodimeric enzyme and participates in several processes such as adapting to hypoxia, histone demethylation, and DNA modification. This enzyme has three isoforms like IDH₁, IDH₂, and IDH₃.[3]

Physical form

Solution in 50% glycerol in EDTA buffer salts, pH 6.0

Other Notes

One unit will convert 1.0 μmole of isocitrate to α-ketoglutarate per min at pH 7.4 at 37 °C.

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Este Item	I1877	M2634	SAB2501739
Sigma-Aldrich I2002 Isocitric Dehydrogenase (NADP) from porcine heart	Sigma-Aldrich I1877 Isocitric Dehydrogenase (NADP) from porcine heart	Sigma-Aldrich M2634 Malic Dehydrogenase from porcine heart	Sigma-Aldrich SAB2501739 Anti-IDH3G antibody produced in goat
specific activity 3-20 units/mg protein	specific activity 0.5-3.0 unit/mg solid (plus numerous enzyme activities associated with porcine heart)	specific activity 600-1000 units/mg protein (biuret)	specific activity -
form buffered aqueous glycerol solution	form solid	form buffered aqueous glycerol solution	form buffered aqueous solution
shipped in wet ice	shipped in -	shipped in wet ice	shipped in wet ice
storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
quality ≤ 0.2% of main activity is obtained with NAD as cofactor.	quality -	quality -	quality -

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Classe de armazenamento

10 - Combustible liquids

wgk

WGK 2

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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