Global chromatin relabeling accompanies spatial inversion of chromatin in rod photoreceptors.

Global chromatin relabeling accompanies spatial inversion of chromatin in rod photoreceptors.

Science advances (2021-09-25)

Cheryl L Smith, Yemin Lan, Rajan Jain, Jonathan A Epstein, Andrey Poleshko

ABSTRACT

[Figure: see text].

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

PD 0325901, ≥98% (HPLC)

Sigma-Aldrich

Bovine Serum Albumin, cold ethanol fraction, pH 5.2, ≥96%

Sigma-Aldrich

Sodium azide, ReagentPlus^®, ≥99.5%

Sigma-Aldrich

CHIR99021, ≥98% (HPLC)

Sigma-Aldrich

Anti-acetyl-Histone H4 Antibody, serum, Upstate^®

Sigma-Aldrich

Propyl gallate, for microscopy, ≥98.0% (HPLC)

Sigma-Aldrich

Gelatin from porcine skin, gel strength 300, Type A

Sigma-Aldrich

ESGRO^® Recombinant Mouse LIF Protein, ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^6 units/ml

Sigma-Aldrich

Anti-acetyl-Histone H3 Antibody, from rabbit

Sigma-Aldrich

DAPI, for nucleic acid staining