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808000

TOYOPEARL® AF-Epoxy-650M Bulk Media

phase expoxy-activated, bottle of 10 g, 65 μm particle size

Synonym(s):

TOYOPEARL® AF-Epoxy-650M Bulk Media

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About This Item

UNSPSC Code:
41115700

product line

TOYOPEARL®

form

powder

packaging

bottle of 10 g

manufacturer/tradename

Tosoh TOYOPEARL® AF, 08000

extent of labeling

800 μeq/g ligand density

parameter

3 bar max. pressure

technique(s)

HPLC: suitable

matrix

hydroxylated methacrylic polymer

matrix active group

expoxy-activated phase

particle size

65 μm

capacity

>60 g/L adsorption capacity (soybean trypsin inhibitor)

separation technique

affinity

storage temp.

2-8°C

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General description

Toyopearl AF-Epoxy-650 resin is an activated resin provided in dry form for the immobilization of protein ligands for affinity chromatography. It is used when high densities of low molecular weight molecules need to be attached. It is also useful when a conversion to other special functional groups is required prior to ligand immobilization. For instance, its hydrazide form is very useful for carbohydrates or glycoprotein ligands.

Application

TOYOPEARL® Affinity Media are used in affinity chromatography, protein chromatography and activated/functionalized matrices.

Physical form

Shipped as dry, free flowing powder.

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Legal Information

Toyopearl is a registered trademark of Tosoh Corporation

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

96.8 °F - closed cup

Flash Point(C)

36 °C - closed cup

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Lot/Batch Number

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D.E.R.™ 332 used as embedding medium

Sigma-Aldrich

31185

D.E.R.™ 332

S Knör et al.
Journal of thrombosis and haemostasis : JTH, 6(3), 470-477 (2008-01-16)
Hemophilia A is currently treated by infusions of the coagulation factor (F) VIII, of which production and purification remain a challenging task. Current purification procedures using immunoaffinity chromatography are cumbersome, expensive, and suffer from the instability of the applied antibody
S Yamauchi et al.
The Journal of biological chemistry, 274(4), 2456-2463 (1999-01-16)
Chondroitin 4-sulfotransferase, which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of N-acetylgalactosamine in chondroitin, was purified 1900-fold to apparent homogeneity with 6.1% yield from the serum-free culture medium of rat chondrosarcoma cells by affinity chromatography on heparin-Sepharose CL-6B, Matrex
Zhenzhen Wen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(20), 1732-1740 (2011-05-17)
In order to improve its stability, immobilized Concanavalin A (Con A) on Toyopearl adsorbents was conjugated with monomethoxy poly(ethylene glycol) succinimidyl propionate (mPEG-SPA) with different molecular weight. A colorimetric method using ninhydrin is proposed to determine the degree of PEGylation;

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