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T1032

Sigma-Aldrich

TNM-FH insect medium

With L-glutamine, without sodium bicarbonate, powder, suitable for insect cell culture

Synonym(s):

Graces Modified Medium

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About This Item

MDL number:
MFCD00243216
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

form

powder

concentration

50.6 g/L

technique(s)

cell culture | insect: suitable

suitability

suitable for culture of other lepidopteran cell lines (Originally developed for the culture of Trichoplusia ni cells)

components

L-glutamine: 0.6 g/L
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

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General description

A number of modifications of Grace′s medium were developed in several laboratories in the late 1960′s and 1970′s. Many of these modifications were developed by Dr. W.F. Hink for the growth of cells derived from the cabbage looper, Tricoplusia ni. This formula represents the modification known as TNM-FH. The medium when properly supplemented has been found to support the growth of cells derived from a variety of lepidopteran species.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S E Crawford et al.
Journal of virology, 68(9), 5945-5952 (1994-09-01)
Rotaviruses are triple-layered particles that contain four major capsid proteins, VP2, VP4, VP6, and VP7, and two minor proteins, VP1 and VP3. We have cloned each of the rotavirus genes coding for a major capsid protein into the baculovirus expression
The baculovirus expression system: a laboratory guide.
King L
Springer Science & Business Media, 2012 null
M Schmidt et al.
The Journal of biological chemistry, 274(21), 14648-14654 (1999-05-18)
Stimulation of phospholipase D (PLD) by membrane receptors is now recognized as a major signal transduction pathway involved in diverse cellular functions. Rho proteins control receptor signaling to PLD, and these GTPases have been shown to directly stimulate purified recombinant
T T Chuang et al.
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H Inohara et al.
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