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SRP0432

Sigma-Aldrich

PDE4D2 active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE)

Synonym(s):

DPDE3, PDE43, STRK1, phosphodiesterase 4D, cAMP-specific

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

Assay

≥80% (SDS-PAGE)

form

aqueous solution

specific activity

≥13600 pmol/min-μg

mol wt

84 kDa

packaging

pkg of 5 μg

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PDE4D(5144)

General description

Human PDE4D2 (GenBank Accession No. NM_001197221) amino acids 2-507 (end) with N-terminal GST-tag, MW=84 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C in a reaction buffer.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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George S Baillie et al.
The Biochemical journal, 404(1), 71-80 (2007-02-10)
Beta2-ARs (beta2-adrenoceptors) become desensitized rapidly upon recruitment of cytosolic beta-arrestin. PDE4D5 (family 4 cAMP-specific phosphodiesterase, subfamily D, isoform 5) can be recruited in complex with beta-arrestin, whereupon it regulates PKA (cAMP-dependent protein kinase) phosphorylation of the beta2-AR. In the present

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