SAB4200828

Anti-Pseudomonas Exotoxin A-Peroxidase antibody produced in rabbit

IgG fraction of antiserum

• Synonym(s): ETA, NAD(+)--diphthamide ADP-ribosyltransferase, PE
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: peroxidase conjugate
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• description: Research area: Microbiome
• form: lyophilized powder
• species reactivity: Pseudomonas aeruginosa
• concentration: ~4 mg/mL
• technique(s): direct ELISA: 1:10,000-1:20,000 (is recommended using 1 μg/ml Pseudomonas Exotoxin A for coating.)
• UniProt accession no.: P11439
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: Pseudomonas aeruginosa PAO1 ... toxA(877850)

Description

General description

Pseudomonas aeruginosa is a Gram negative bacterium that rarely cause diseases in humans but certain populations, such as cystic fibrosis (CF) or burn wounds patients, are prone to such infections.
Pseudomonas Exotoxin A (PE) is the most potent virulence factor secreted by P. aeruginosa, it is composed of three structural domains, N-terminal domain (I) responsible for the toxin binding to its host cell receptor, middle domain (II) has a role in toxin translocation across the membrane, and C-terminal domain (III) has ADP-ribosylation activity. PE is considered as a selective agent for the elimination of specific cell populations due to its toxin ADP-ribosylation activity resulting in irreversibly shut down of protein synthesis. To reduce the adverse effects of natural PE, Mutated PE was used in several attempts to develop recombinant toxin-antibody or cytokines fusion fragments for therapeutic application including cancer immunotherapy.

Immunogen

Purified Exotoxin A from Pseudomonas aeruginosa

Application

The antibody may be used in various immunochemical techniques including ELISA.

Physical form

Supplied as a lyophilized powder.

Other Notes

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable