Recommended Products
form
suspension
particle size
20-40 μm (wet)
pore size
1,000-300,000 Da fractionation range (globular proteins)
storage temp.
2-8°C
Application
Superose® 12 prep grade is used for protein chromatography, gel filtration chromatography and gel filtration media. Superose® 12 prep grade has been used to purify and characterize a haemolysin of Actinomyces pyogenes as well as a fibrinogenase from Vipera lebetina (desert adder) venom. Superose® 12 prep grade has also been used for the isolation and characterization of an extracellular protease of Actinomyces pyogenes.
Other Notes
Highly cross-linked beaded agarose
Physical form
Suspension in 20% ethanol
aqueous ethanol suspension
Legal Information
Superose is a registered trademark of Cytiva
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
Flash Point(F)
100.4 - 109.4 °F
Flash Point(C)
38 - 43 °C
Certificates of Analysis (COA)
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A Gasmi et al.
Toxicon : official journal of the International Society on Toxinology, 29(7), 827-836 (1991-01-01)
A fibrinogenase from Vipera lebetina venom was isolated by gel filtration in a Superose 12 column prep grade HR 16/50 and by ion-exchange in a Mono Q HR 5/5 column. The purified enzyme, which was obtained with a yield of
Shih-Chieh Lee et al.
Journal of agricultural and food chemistry, 52(16), 4948-4952 (2004-08-05)
Our research on several proteins indicates that accurate molecular weights cannot be determined by Superose 12 column chromatography. In support of this statement, we present data on molecular weights of purified red kidney bean alpha-amylase inhibitor (RKB alphaAI) and white
G A Ameer et al.
Kidney international, 59(4), 1544-1550 (2001-03-22)
High plasma levels of beta2-microglobulin (beta2m) have been implicated in the formation of the severely destructive and potentially fatal amyloid deposits that are characteristic of dialysis-related amyloidosis (DRA). Conventional renal replacement technologies remove insufficient quantities of beta2m to normalize plasma
Jun Xu et al.
Journal of chromatography. A, 1169(1-2), 235-238 (2007-09-28)
(-)-Epigallocatechin gallate (EGCG) was purified in one step from a green tea polyphenol (GTP) crude extract by adsorption chromatography on a Superose 12 HR 10/30 column. The mobile phase used was a mixture of acetonitrile and water with an optimum
A Van Tol et al.
Journal of lipid research, 32(11), 1719-1728 (1991-11-01)
The present study demonstrates very high levels of plasma lipids and high density lipoprotein (HDL) apolipoproteins (apoA-I and apoE) in female Nagase analbuminemic rats (NAR) fed a semi-synthetic diet in order to further increase the hyperlipidemia present in this strain.
Articles
This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.
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