The kit MAK157 does not contain any Renin inhibitor. To confirm the activity using this kit, researchers can use a RENIN INHIBITOR separately.The amount of inhibitor to use and the step to use the Renin inhibitor can be referred to from published literature.
MAK157
Renin Assay Kit
Renin Assay Kit
1 of 1 reviewers received a sample product or took part in a promotion
sufficient for 100 fluorometric tests
Synonym(s):
Renin Enzyme Detection Kit
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1 KIT
R$1,807.00
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 fluorometric tests
detection method
fluorometric
relevant disease(s)
cardiovascular diseases
storage temp.
−20°C
Gene Information
human ... REN(5972)
rat ... REN(24715)
Related Categories
General description
Renin, also known as angiotensinogenase, is an aspartyl protease that cleaves angiotensinogen to form angiotensin I, the initial and rate-limiting step of the renin-angiotensin system (RAS). This system plays a critical role in the regulation of blood pressure and fluid balance. Increased renin activity can result in hypertension due to increased vasoconstriction and fluid retention. Inhibitors of renin can be used as a treatment for hypertension.[1]
Suitability
The Renin Assay Kit provides a convenient and simple method for screening renin activity in a variety of samples or for screening renin inhibitors.
Principle
Renin activity is determined using a proprietary fluorescence resonance energy transfer (FRET) peptide containing a fluorescence quencher. Cleavage of the peptide by renin results in the production of a fluorescent product (λex = 540/λem = 590 nm). This assay is ~50-fold more sensitive than the EDANS/DABCYL-based assay.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Does the kit MAK157 contain any Renin inhibitor? If so, how much Renin inhibitor should be added, and at what step of the assay should it be added in order to determine the Renin activity?
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