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MAK064

Sigma-Aldrich

Lactate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Lactic Acid Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

endocrinological disorders, diabetes; cancer

storage temp.

−20°C

General description

L(+)-Lactate is a metabolic compound formed in animals by the action of the enzyme Lactate Dehydrogenase. Lactate is produced in proliferating cells and during anaerobic conditions such as exercise. Abnormally high concentrations of lactate have been related to pathological conditions such as cancer, diabetes, and lactate acidosis. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood at levels of around 1-2 mmol/L. This kit provides a convenient means for detecting L(+)-Lactate in biological samples. There is no need of pretreatment or purification of samples.

Application

Lactate Assay Kit has been used to determine the concentrations of lactate in samples.

Suitability

Suitable for detecting L(+)-Lactate in culture medium, fermentation medium, blood and cells.

Principle

In this assay, lactate concentration is determined by an enzymatic assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the lactate present. Typical levels of sensitivity for this kit are between 0.001-10 mM.

replaced by

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Min Xu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 47(1), 151-160 (2018-05-16)
Poor response to chemotherapy leads to the relapse and metastatic progression of tumors. Reprogrammed glucose metabolism is one of the important hallmarks of cancer that facilitates cancer cell survival, proliferation and chemoresistance. However, the precise fate of glucose metabolism and
Prabha Shrestha et al.
PLoS pathogens, 13(9), e1006628-e1006628 (2017-09-19)
Primary effusion lymphoma (PEL) is an aggressive B-cell lymphoma with poor prognosis caused by Kaposi's sarcoma-associated herpesvirus (KSHV). Previous studies have revealed that HIF-1α, which mediates much of the cellular response to hypoxia, plays an important role in life cycle
Bibi Uhre Nielsen et al.
PloS one, 15(9), e0238524-e0238524 (2020-09-12)
Markers of lung inflammation measured directly in expectorated sputum have the potential of improving the timing of antibiotic treatment in cystic fibrosis (CF). L-Lactate might be a marker of inflammation, as it is produced from glucose by polymorphonuclear neutrophils (PMNs)
Cardiac STAT3 Deficiency Impairs Contractility and Metabolic Homeostasis in Hypertension.
Altara R, et al.
Frontiers in Pharmacology, 7, 436-436 (2016)
Ivan Kushkevych et al.
Journal of clinical medicine, 8(7) (2019-07-25)
The small-large intestine axis in hydrogen sulfide accumulation and testing of sulfate and lactate in the gut-gut axis of the intestinal environment has not been well described. Sulfate reducing bacteria (SRB) of the Desulfovibrio genus reduce sulfate to hydrogen sulfide
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