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M6774

Sigma-Aldrich

McCown′s Woody Plant Basal Salt Mixture

powder, suitable for plant cell culture

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.72

form

powder

Quality Level

200

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

Related Categories

General description

McCown′s Woody Plant Basal Salt Mixture is the non-vitamin component (chemstock) of the Woody Plant Medium (WPM) is used for propagation and elongation of root cultures.

Application

McCown′s Woody Plant Basal Salt Mixture has been used to culture apical and axillary buds. It has also been used to subculture individual shoots for the establishment of shoot tips in tissue culture.

Formula variant

With the macro- and micronutrients as described by Lloyd and McCown (1981).

Media Formulation

Quantity

Formulated to contain 2.3 grams of powder per liter of medium.

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Product No.
Description
Pricing

Signal Word

Danger

Hazard Statements

H272,H302,H318

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Ox. Sol. 3

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Ercan Arican et al.
Journal of environmental biology, 29(6), 841-844 (2009-03-21)
This study was conducted to explain difficulties of indirect regeneration of forest trees in tissue culture conditions. For this purpose, changes of antioxidant defense system enzymes; superoxide dismutase (SOD), peroxidase (POD) activities were determined during calli formation on young apical
Swapna R Deodhar et al.
Indian journal of experimental biology, 46(6), 482-486 (2008-08-14)
Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medium supplemented with BAP (0.44-2.22 microM) showed production of de novo shoots. Roots attached to mother plant showed more number of shoots, while excised root
Commercially-feasible micropropagation of Mountain Laurel, Kalmia latifolia, by shoot tip culture.
Lloyd, G., and McCown, B.H.
Proc. Int. Plant Prop. Soc., 30, 421-427 (1981)
Enhanced micropropagation and establishment of grapevine rootstock genotypes
Alzubi H, et al.
International Journal of Plant Developmental Biology, 6(1), 9-14 (2012)
Guo-Qing Song et al.
Plant cell reports, 25(2), 117-123 (2005-12-22)
Sour cherry (Prunus cerasus L.) scion cv. Montmorency and rootstock cv. Gisela 6 (P. cerasus x P. canescens) were transformed using Agrobacterium tumefaciens strain EHA105:pBISN1 carrying the neomycin phosphotransferase gene (nptII) and an intron interrupted ss-glucuronidase (GUS) reporter gene (gusA).
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Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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