No, if using a 95% ethanol step before eosin staining, it is important to avoid dragging water into the alcoholic eosin solution. Introducing too much water might make the solution more alkaline, which can affect the staining. pH is not typically measured as it tends to stain the pH electrode. Additionally, because this is a colored solution, using pH papers is not a viable option. If staining is poor, the solution either needs to be replaced, or it is possible to add 0.5 mL of glacial acetic acid per 100 ml of eosin Y solution to improve the staining.
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form
solution
Quality Level
shelf life
Expiry date on the label.
IVD
for in vitro diagnostic use
concentration
0.5 % (w/v) in acidified ethanol
application(s)
hematology
histology
storage temp.
room temp
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Application
General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
Other Notes
Certified Eosin Y, 0.5% (w/v) in acidified 90% ethanol
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Sens. 1
Storage Class Code
3 - Flammable liquids
WGK
WGK 2
Flash Point(F)
55.4 °F - closed cup
Flash Point(C)
13 °C - closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Does the Eosin Y alcoholic solution (HT1101) need to be pH tested regularly?
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