H7414

Anti-HIP14 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-DHHC domain containing 17 (ZDHHC17), Anti-HSPC294, Anti-Huntingtin interacting protein 3 (HIP3), Anti-Huntingtin interacting protein H (HYPH), Anti-Huntingtin-Interacting Protein 14, Anti-KIAA0946, Anti-Zinc finger
• MDL number: MFCD09265361
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~75 kDa (mouse); antigen ~78 kDa (human)
• species reactivity: mouse, canine, human, rat, bovine
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): western blot: 0.5-1 μg/mL using extracts of HEK-293T cells expressing human HIP14 and mouse brain extracts (P2 membrane fraction)
• UniProt accession no.: Q8IUH5
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ZDHHC17(23390) ; mouse ... Zdhhc17(320150)

Description

General description

Huntingtin interacting protein 14 (HIP14) is a palmitoyl transferase (PAT) neuronal type, localizes to the Golgi, and to vesicles in the cytoplasm. HIP14 mRNA is predominantly expressed in the brain.

Immunogen

synthetic peptide corresponding to amino acids 614-632 located at the C-terminus of human HIP14. This sequence is identical in mouse, rat, dog, and bovine HIP14.

Application

Anti-HIP14 antibody produced in rabbit has been used in quantitative immunoblotting, immunohistochemistry.

Biochem/physiol Actions

The interaction of huntingtin interacting protein 14 (HIP14) with huntingtin protein (htt) is inversely correlated to the polyQ length.. HIP14 along with htt is localized in striatum, medium spiny projection neurons. It is also associated neurons associated with Huntington disease (HD). HIP14 under expression in neurons reduces the clustering of postsynaptic density protein 95 (PSD-95) and glutamic acid decarboxylase 65-kilodalton isoform (GAD65). It has been suggested that HIP14 has a role in palmitoylation and in regulating the intracellular trafficking of multiple neuronal proteins. Decreased interaction between mutant htt and HIP14 could contribute to neuronal dysfunction in HD by perturbing normal intracellular transport pathways in neurons.

Target description

HIP14 (also known as zinc finger, DHHC domain containing protein 17 (ZDHHC17), huntingtin interacting protein H (HYPH), huntingtin interacting protein 3 (HIP3), HSPC294 and KIAA0946) is a novel htt interacting protein.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves