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swelling
-1 g swells to 9-11 mL
bead size
100-300 μm
InChI
1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2
InChI key
FZWBNHMXJMCXLU-UHFFFAOYSA-N
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Application
Sephadex® G-50 is a gel filtration medium used in affinity chromatography, protein chromatrography and gel filtration chromatography. Sephadex® G-50 has been used to investigate effects from snake venom, isolate lectins from various species and to demonstrate the positive influence of 20% ethanol on proteinase desorption.
Legal Information
Sephadex is a registered trademark of Cytiva
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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I Shelub et al.
Journal of applied physiology: respiratory, environmental and exercise physiology, 56(3), 810-815 (1984-03-01)
Despite numerous efforts, a reliable model of chronic embolic pulmonary hypertension has not been established. To develop such a model five conscious mongrel dogs were embolized repeatedly over 16-30 wk with Sephadex microspheres 286 +/- 70 micron in diameter. Hemodynamic
Martin Jaburek et al.
The Journal of biological chemistry, 279(51), 53097-53102 (2004-10-12)
Functional activation of mitochondrial uncoupling protein-2 (UCP2) is proposed to decrease reactive oxygen species production. Skulachev and Goglia (Skulachev, V. P., and Goglia, F. (2003) FASEB J. 17, 1585-1591) hypothesized that hydroperoxy fatty acid anions are translocated by UCPs but
Li-Feng Huang et al.
Toxicon : official journal of the International Society on Toxinology, 51(6), 1008-1016 (2008-02-19)
A group of small proteins, designated as muscarinic toxins (MTs), have been isolated from the venom of African green mamba (Dendroaspis angusticeps) and documented to bind selectively to individual muscarinic acetylcholine receptor (mAChR) subtypes. These components have less been reported
Tomohiro Suzuki et al.
Glycobiology, 22(5), 616-629 (2011-12-27)
A lectin was purified from the mushroom Hygrophorus russula by affinity chromatography on a Sephadex G-50 column and BioAssist S cation exchange chromatography and designated H. russula lectin (HRL). The results of sodium dodecyl sulfate-polyaclylamidegel electrophoresis, gel filtration and matrix-assisted
U R Radzhabov et al.
Prikladnaia biokhimiia i mikrobiologiia, 47(3), 272-276 (2011-07-28)
Abstract-A simple and efficient method of preparing highly purified extracellular proteinases of B. subtilis B-1 (SKB 256) has been developed. A sorbent based on sorsilen impregnated with hemoglobin or cytochrome c has been synthesized for this purpose. A significant difference
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