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F9665

Sigma-Aldrich

Fetal Bovine Serum

Heat Inactivated, non-USA origin, sterile-filtered, suitable for cell culture

Synonym(s):

HI FBS, HI FCS, HI Fetal Calf Serum, HI Serum, HI sera, Heat Inactivated Fetal Calf Serum, FBS, FCS, sera, serum

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.71

biological source

bovine fetus

Quality Level

sterility

sterile-filtered

product line

FBS Classic

composition

Hemoglobin, ≤25 mg/dL

origin

non-USA origin

availability

not available in USA

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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Application

FBS is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.

Preparation Note

Prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes is used to inactivate the complement system for immunoassays. Heat inactivation also inactivates other undetermined inhibitors of cell growth in culture.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Legal Information

Not available in USA or Canada. Please check with your local supplier regarding availability and import restrictions in your country.

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Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Karoline Pill et al.
Frontiers in bioengineering and biotechnology, 6, 156-156 (2018-11-10)
A promising approach to overcome hypoxic conditions in tissue engineered constructs is to use the potential of endothelial cells (EC) to form networks in vitro when co-cultured with a supporting cell type in a 3D environment. Adipose tissue-derived stromal cells
Amina Kamar et al.
Frontiers in genetics, 8, 217-217 (2018-01-13)
Despite an obvious role for consanguinity in congenital heart disease (CHD), most studies fail to document a monogenic model of inheritance except for few cases. We hereby describe a first-degree cousins consanguineous Lebanese family with 7 conceived children: 2 died
Xiaolu Zhang et al.
Nature communications, 10(1), 4732-4732 (2019-10-20)
Current multiplexing strategies for massively parallel sequencing of genomic DNA mainly rely on library indexing in the final steps of library preparation. This procedure is costly and time-consuming, because a library must be generated separately for each sample. Furthermore, library
Athar Khalil et al.
Frontiers in oncology, 8, 482-482 (2018-11-15)
T-box (TBX) transcription factors are evolutionary conserved genes and master transcriptional regulators. In mammals, TBX2 subfamily (TBX2, TBX3, TBX4, and TBX5) genes are expressed in the developing lung bud and tracheae. Our group previously showed that the expression of TBX2
Aintzane García-Bea et al.
Journal of psychopharmacology (Oxford, England), 31(12), 1519-1526 (2017-06-29)
As well as being expressed as a full-length transcript, the group II metabotropic glutamate receptor 3 (GRM3, mGlu3) gene is expressed as an mRNA isoform which lacks exon 4 (GRM3Δ4) and which is predicted to encode a protein with a

Articles

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

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