BE10-Late
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
biological source
rat brain
packaging
tube of 5 μg 93040120-DNA-5UG
pkg of vial of cells 93040120-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Plasminogen activator activity
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat, BDIX, foetal brain, malignant
Cell Line Description
Malignant cells produced on passaging BE10-Intermediate (Sigma Catalogue number. 93040119) beyond passage 40. The cells possess tissue plasminogen activator activity, are tumourigenic in BDIX rats and form colonies in agar.
Application
Study of carcinogenesis, tumourigenicity studies
Culture Medium
DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-2x100,000 cells/25cm2 using 0.25% trypsin/EDTA; 10-15% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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