Derived from the parent line CHO-K1 and is hypersensitive to ionising radiation but not to UV irradiation. Cells may revert to wild-type after 1-2 weeks in culture. They should be checked for sensitivity and recloned when necessary.
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
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