Derived from kidney of a vervet monkey. Distribution restricted to non-profit organisations with involvement in virus vaccine research and development.
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
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