L-M (TK-)
90083001, mouse connective tissue, Fibroblast
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About This Item
UNSPSC Code:
41106514
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Product Name
L-M (TK-), 90083001
biological source
mouse connective tissue
growth mode
Adherent
karyotype
Modal no. 45; hyperdiploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse C34/An connective tissue
Cell Line Description
Derived from a sub-line of a BUdR resistant strain of the L-M mouse fibroblast cell line. This cell line has potential for use in genetic and biochemical studies including somatic cell hybridisation and gene transfer.
Application
Gene transfer, genetic, biochemical and hybridisation studies
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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