K-BALB (K-234)

89092101, mouse embryo, Fibroblast

• UNSPSC Code: 41106514

Properties

• Product Name: K-BALB (K-234), 89092101, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• biological source: mouse embryo
• packaging: tube of 5 μg 89092101-DNA-5UG; pkg of vial of cells 89092101-1VL
• growth mode: Adherent
• karyotype: 2n=40
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse A31 embryo Kirsten sarcoma, transformed

Cell Line Description

Cell morphology is different from the parent clone (A31) and there is a loss of sensitivity to contact inhibition of cell division. The cells are highly tumorigenic. Superinfection with MuLV results in rescue of MSV.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.1% trypsin for 3-5 minutes; 5% CO₂; 37°C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.