H9

85050301, human blood, Lymphoblast

• UNSPSC Code: 41106514

Properties

• product name: H9, 85050301
• biological source: human blood
• growth mode: Suspension
• karyotype: Modal no. 69
• morphology: Lymphoblast
• products: CD3, CD4
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human T cell

Cell Line Description

Clonal derivative of the T-cell line HUT 78 derived from the blood of a patient with Sezary syndrome. The clone was selected due to its suitability for the isolation and continuous high level production and HIV variants with patients within AIDS and pre-AIDS. Examination with DNA probes indicate identical HLA typing and DNA fingerprints as HUT-78 .

Application

Detection, isolation and propagation of HIV-1

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,12
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9
TPOX: 8,9
vWA: 14,15

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

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