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C7464

Sigma-Aldrich

Monoclonal Anti-Cyclin D1 antibody produced in mouse

clone DCS-6, ascites fluid

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About This Item

MDL number:
MFCD00239664
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

DCS-6, monoclonal

mol wt

antigen 36 kDa (several lower and higher molecular weight bands)

contains

15 mM sodium azide

species reactivity

rat, mouse, human, monkey

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:200 using a human tumor cell line extract

isotype

IgG2a

UniProt accession no.

P24385

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CCND1(595)
mouse ... Ccnd1(12443)
rat ... Ccnd1(58919)

General description

Monoclonal Anti-Cyclin D1 (mouse IgG2a isotype) is derived from the DCS-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cyclin D1 protein is a G1-phase cyclin in mammalian cells. This protein is a D-type cyclin. The CCD1 gene is located on the human chromosome at 11q13.3.

Specificity

Does not cross-react with other D-type cyclins.

Immunogen

recombinant human Cyclin D1 protein.

Application

Monoclonal Anti-Cyclin D1 antibody produced in mouse may be used in:
  • immunoblotting
  • immunofluorescence
  • immunocytochemistry
  • immunoprecipitation
  • immunohistochemistry
  • antibody-mediated knock-out experiments

Biochem/physiol Actions

Cyclin D1 is involved in regulating cyclin-dependent kinase (CDK) family members. Cyclin D1 and D2 are affiliated with CDK4 and/or CDK6 kinase activities that are observed in mid-G1 and culminate in the late G1 phase. Amplification of the cyclin D1 gene is observed in a significant percentage of cancers, including breast, bladder carcinoma, mantle cell lymphomas, head and neck cancers. This gene is also amplified in esophageal squamous cell carcinomas.

Physical form

The product is provided as ascites fluid with 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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