C3735
Cytochrome P450 human
1A1 Isozyme Microsomes, with P450 Reductase, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
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About This Item
MDL number:
UNSPSC Code:
12161501
NACRES:
NA.47
Recommended Products
biological source
human
Quality Level
recombinant
expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
form
solution
specific activity
≥4 units/pmol enzyme
mol wt
45-60 kDa
packaging
vial of 0.5 nmol
technique(s)
activity assay: suitable
solubility
water: soluble
suitability
suitable for molecular biology
UniProt accession no.
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CYP1A1(1543)
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General description
Research area: Immuno and CKS
Cytochrome P450 (CYP) enzymes are a family of enzymes that are encoded by the P450 genes. These enzymes are membrane bound hemoproteins. that are mainly found in the liver’s endoplasmic reticulum.
Cytochrome P450 (CYP) enzymes are a family of enzymes that are encoded by the P450 genes. These enzymes are membrane bound hemoproteins. that are mainly found in the liver’s endoplasmic reticulum.
Biochem/physiol Actions
Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous. The CYP1A1 isoform catalyzes 7-deethylation of ethoxyresorufin. Cytochrome P450 (CYP) plays an important role in detoxifying xenobiotics, cellular metabolism and homeostasis. One of the main mechanisms of drug-drug interactions is the induction or inhibition of these enzymes. CYP enzymes are transcriptionally activated by a variety of xenobiotics and by endogenous substrates via receptor-dependent pathways. Inhibition of these enzymes is a major factor in metabolism-based drug-drug interactions, and many chemotherapeutic medications can cause drug interactions by either inhibiting or inducing the cytochrome p450 enzyme system.
Physical form
Solution in 100 mM potassium phosphate buffer, pH 7.4.
Preparation Note
Microsomes containing human CYP1A1 and recombinant human NADPH-P450 reductase.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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