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A3653

Sigma-Aldrich

Actin from bovine muscle

lyophilized powder

Synonym(s):

G-Actin

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About This Item

CAS Number:
51005-14-2
MDL number:
MFCD00130338
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

bovine muscle

Quality Level

200

Assay

≥90% (SDS-GE)

form

lyophilized powder

mol wt

43 kDa

composition

Protein, ≥80% biuret

technique(s)

western blot: suitable

UniProt accession no.

P62739

storage temp.

2-8°C

Gene Information

bovine ... LOC(528168)

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General description

Actin is a highly abundant and conserved protein. It can transition between the monomeric (G-actin) and filamentous (F-actin) forms, which is influenced by external factors. Actin is expressed in smooth, skeletal and cardiac muscles.

Application

Actin from bovine muscle has been used as an internal standard and positive control during western blotting.

Biochem/physiol Actions

Actin is involved in a wide variety of cellular processes like cell migration, phagocytosis, cell polarity and shape. It associates with myosin and aids in muscle contraction. The protein acts as a building block of microfilaments.

Physical form

Lyophilized powder containing Tris, ATP and CaCl2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Actin Protein

Actin isoforms are muscle tissue markers; six isoforms expressed, four for muscle differentiation, two ubiquitous.

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