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75925

Oxonol V

Name: Oxonol V
Brand: SIGMA

for fluorescence, ≥95% (UV)

Synonym(s):

1,5-Penta-1,3-dien-1-yl-5-ylidene-bis(5-oxo-3-phenyl-4-isoxazole), 4-[5-(5-Hydroxy-3-phenyl-4-isoxazolyl)-2,4-pentadienylidene]-3-phenyl-5(4H)-isoxazolone

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About This Item

Empirical Formula (Hill Notation):

C₂₃H₁₆N₂O₄

CAS Number:

61389-30-8

Molecular Weight:

384.38

Beilstein:

575970

MDL number:

MFCD00075232

UNSPSC Code:

12171500

PubChem Substance ID:

24886894

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grade

for fluorescence

Assay

≥95% (UV)

suitability

in accordance for fluorescence

shipped in

wet ice

storage temp.

−20°C

SMILES string

Oc1onc(-c2ccccc2)c1\C=C\C=C\C=C3/C(=O)ON=C3c4ccccc4

InChI

1S/C23H16N2O4/c26-22-18(20(24-28-22)16-10-4-1-5-11-16)14-8-3-9-15-19-21(25-29-23(19)27)17-12-6-2-7-13-17/h1-15,26H/b9-3+,14-8+,19-15-

InChI key

NEDZCQDBZLHEGJ-UFRBDIPQSA-N

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Other Notes

Potential sensitive probe. Plasma membrane potential of neutrophils generated by the Na⁺ pump[1]; Spectral properties[2]

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Plasma membrane potential of neutrophils generated by the Na+ pump.

C L Bashford et al.

Biochimica et biophysica acta, 817(1), 174-180 (1985-07-11)

The plasma membrane potential of human neutrophils was monitored using the anionic dye oxonol-V. The cells maintain a potential of -75 +/- 17 mV when suspended in physiological saline solutions. The cells are scarcely depolarized by extracellular K+ and the

Synthesis, structure determination, spectral properties, and energy-linked spectral responses of the extrinsic probe oxonol V in membranes.

J C Smith et al.

Biochemistry, 15(23), 5094-5105 (1976-11-16)

The direct synthesis of bis]3-phenyl-5-oxoisoxazol-4-yl]pentamethineoxonol, which is shown to be the fluorescent probe OX-V (formerly MC-V), is described. The emission lifetime (0.9 +/- 0.1 ns) and the spectral properties of this dye in a number of systems are presented as

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