Sybr Green and Fluorescein sodium salt are both green dyes, and although their spectral ranges are close, it is important to consider their specific uses and characteristics. While Sybr Green is a popular dye for molecular use, Fluorescein sodium salt is a very economical product and may be useful for specific applications. However, there is no information comparing the two dyes for use in PCR.
46960
Fluorescein sodium salt
BioReagent, suitable for fluorescence
Synonym(s):
Acid Yellow 73, NaFl, Sodium fluorescein, Yellow No. 8, D&C;Yellow No. 8, NaFluo, Uranine
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25 G
R$187.00
100 G
R$495.00
500 G
R$1,514.00
About This Item
Empirical Formula (Hill Notation):
C20H10Na2O5
CAS Number:
Molecular Weight:
376.27
Colour Index Number:
45350
Beilstein:
3833041
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.32
Recommended Products
product line
BioReagent
Quality Level
form
powder
technique(s)
titration: suitable
fluorescence
λex 460 nm; λem 515 nm(lit.)
λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0
suitability
suitable for fluorescence
SMILES string
[Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1
InChI
1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2
InChI key
RGPLGPBQJOQFJS-UHFFFAOYSA-L
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General description
Fluorescein Sodium Salt (FSS) is a xanthene dye having a polycrystalline structure with a monoclinic system. Fluorescein is a synthetic organic compound available as a dark orange/red powder soluble in water and alcohol.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
423.7 °F - Pensky-Martens closed cup
Flash Point(C)
217.6 °C - Pensky-Martens closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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For Fluorescein sodium salt 46960, is there a fluorescence value for a positive PCR sample, and how would this value translate as a concentration of this powder?
1 answer-
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