650498

Chloroform

HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer

• Synonym(s): Methylidyne trichloride, Trichloromethane
• Empirical Formula (Hill Notation): CHCl₃
• CAS Number: 67-66-3
• Molecular Weight: 119.38
• Beilstein: 1731042
• EC Number: 200-663-8
• MDL number: MFCD00000826
• UNSPSC Code: 12190000
• PubChem Substance ID: 329760024
• NACRES: NA.04

Properties

• grade: HPLC Plus; for residue analysis
• vapor density: 4.1 (vs air)
• vapor pressure: 160 mmHg ( 20 °C)
• Assay: ≥99.9%
• form: liquid
• contains: amylenes as stabilizer
• technique(s): HPLC: suitable; gas chromatography (GC): suitable
• impurities: ≤0.02% water; ≤0.5 ppb Fluorescence (quinine) at 365 nm
• evapn. residue: ≤0.0001%
• halogenated residue: <10 ng/L (as heptachlor epoxide)
• refractive index: n20/D 1.445 (lit.)
• bp: 60.5-61.5 °C (lit.)
• mp: −63 °C (lit.)
• density: 1.48 g/mL at 25 °C; 1.492 g/mL at 25 °C (lit.)
• λ: H₂O reference
• UV absorption: λ: 245 nm A_max: 1.00; λ: 250 nm A_max: 0.30; λ: 275-400 nm A_max: 0.005
• application(s): food and beverages
• SMILES string: ClC(Cl)Cl
• InChI: 1S/CHCl3/c2-1(3)4/h1H
• InChI key: HEDRZPFGACZZDS-UHFFFAOYSA-N

Description

General description

Chloroform is an organic chlorinated hydrocarbon (CHC) commonly used in industries as a solvent.

Application

Chloroform may be used in the following:

• Extraction of active constituents of anti-inflammatory drugs for their analysis by attenuated total reflectance-Fourier transform infrared spectroscopic (ATR-FTIR) methods
• Estimation of ascorbic acid, free phenolic acids, and flavonoids in extracts of chloroform, methanol, water and, acetic acid obtained from two edible leaf samples using reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to photodiode array detector (DAD)
• Preparation of cannabis product extracts for the analysis of delta-9-tetrahydrocannabinol (THC) residues on hands of suspected consumers by ion-mobility spectrometry (IMS)
• Investigation of different solvents to measure their recovery ratios for the extraction of acrylamide and 5-hydroxymethylfurfural (HMF) from seafood samples to develop an HPLC-MS/MS-based method for their quantification
• Extraction of pesticides by direct-immersion single drop micro-extraction (SDME) method from mango samples for their subsequent analysis by GC-MS
• Sample preparation in the profiling of lipids from complex biological samples using off-line mixed-mode liquid chromatography coupled with reversed phase high performance liquid chromatography-high resolution mass spectrometry

Chloroform was used in the making of silanized coverslips for DNA combing.
It may be used as:

• Extractant in solvent extraction process.
• A component of the binary mobile phases for liquid chromatography–atmospheric pressure photoionization-mass spectrometry (LC–APPIMS) of triacylglycerol model compounds.
• Solvent for recrystallization.

Suitable for HPLC, spectrophotometry, environmental testing

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Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H302,H315,H319,H331,H336,H351,H361d,H372
• Precautionary Statements: P202 - P301 + P312 - P302 + P352 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3
• Target Organs: Central nervous system, Liver,Kidney
• Storage Class Code: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 3
• Flash Point(F): does not flash
• Flash Point(C): does not flash