PPB020
SSC Buffer
pH 7.0, pHast Pack™, powder
Synonym(s):
1X SSC buffer, 1X SSC hybridization buffer, SSC hybridization buffer, Saline-sodium citrate buffer
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Application
Saline sodium citrate buffer is commonly used as a wash buffer for Nucleic acid hybridization techniques like Northern and Sothern blotting.
Features and Benefits
- Ready-to-use buffer
- No measuring and pH adjusting needed
- Save time and minimize effort
- Biological tests: free of DNase, RNase, protease, and nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
Packaging
Foil pouches
Reconstitution
Contents of one pouch, when dissolved in 1000 mL of ultrapure water, will yield a 1× solution containing 150 mM NaCl and 15 mM Na3C6H5O7, pH 7.0 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.
Storage and Stability
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
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