Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

12125

Sigma-Aldrich

Benzidine dihydrochloride

for experimental carcinogenesis, ≥99.0%

Synonym(s):

4,4′-Diaminobiphenyl dihydrochloride

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C12H12N2 · 2HCl
CAS Number:
531-85-1
Molecular Weight:
257.16
Beilstein:
3914846
EC Number:
208-519-6
MDL number:
MFCD00040389
UNSPSC Code:
12352207
PubChem Substance ID:
329749630
NACRES:
NA.25

grade

for experimental carcinogenesis

Quality Level

100

Assay

≥99.0% (AT)
≥99.0%

form

solid

ign. residue

≤0.05%

mp

≥300 °C

SMILES string

Cl[H].Cl[H].Nc1ccc(cc1)-c2ccc(N)cc2

InChI

1S/C12H12N2.2ClH/c13-11-5-1-9(2-6-11)10-3-7-12(14)8-4-10;;/h1-8H,13-14H2;2*1H

InChI key

RUAXWVDEYJEWRY-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

Benzidine dihydrochloride (BDHC) may be used as a chromogenic substrate for the detection of horseradish peroxidase in immunochemical and immunohistochemical applications.

Warning

Cancer suspect agent.

Other Notes

Indicator of carcinogenicity in bioassay studies[1]; For the detection of horseradish peroxidase in histochemical studies[2][3]
Sales restrictions may apply

Signal Word

Danger

Hazard Statements

H302,H350,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1A

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
WE314499

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

S Lakos et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 34(8), 1047-1056 (1986-08-01)
Although very sensitive chromogens have been adapted for localization of horseradish peroxidase in anterograde and retrograde tracing studies, they have not been successfully applied in immunocytochemical studies. This report describes a protocol which uses benzidine dihydrochloride (BDHC) as the chromogen
C.H. Frith et al.
Toxicology Letters, 4, 507-507 (1979)
M M Mesulam et al.
Brain research, 136(3), 393-414 (1977-11-18)
The caudal part of the inferior parietal lobule (area PG) was injected with horseradish peroxidase (HRP) in 6 hemispheres of 5 rhesus monkeys. The retrograde transport of HRP resulted in the labeling of neurons in diverse cortical and subcortical areas.
M M Mesulam et al.
Neuroscience letters, 5(1-2), 7-14 (1977-06-01)
Four rhesus monkeys and two rats were injected with horseradish peroxidase (HRP). Adjacent sections were stained with a 3,3'-diaminobenzidine tetrahydrochloride (DAB) and a benzidine dihydrochloride (BDHC) procedure which yields a blue reaction product at sites of HRP activity. The number
Ping Lin et al.
Molecular cell, 78(5), 850-861 (2020-04-30)
Cas13 has demonstrated unique and broad utility in RNA editing, nucleic acid detection, and disease diagnosis; however, a constantly active Cas enzyme may induce unwanted effects. Bacteriophage- or prophage-region-encoded anti-CRISPR (acr) gene molecules provide the potential to control targeting specificity
View All Related Papers

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service