01337

Mitis Salivarius Agar

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): M-S Agar
• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15.0 g/L ; casein enzymatic hydrolysate, 15.0 g/L ; crystal violet, 0.0008 g/L ; dextrose, 1.0 g/L ; dipotassium phosphate, 4.0 g/L ; peptic digest of animal tissue, 5.0 g/L ; sucrose, 50.0 g/L ; trypan blue, 0.075 g/L
• manufacturer/tradename: NutriSelect^® Plus
• final pH: 7.0±0.2 (25 °C)
• application(s): clinical testing; food and beverages; veterinary; microbiology
• suitability: selective and differential for Enterococcus spp.; selective and differential for Streptococcus spp.

Description

Application

Mitis Salivarius Agar is recommended for the isolation from mixed cultures of Streptococci especially Streptococcus mitis, Streptococcus salivarius, Enterococcus faecalis showing α and γ haemolytic reactions on Blood Agar.

Preparation Note

Suspend 90 g in 1000 ml distilled water. Boil to dissolve the medium completely. Dispense and sterilize by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and add 1 ml of sterile 1 % potassium tellurite solution (Cat. No. 17774). DO NOT REHEAT the medium after the addition of tellurite solution.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves